MECANISMOS MOLECULARES:
POSIBLE IMPLICACIÓN DEL GENRNASEH1 EN LA
ETIOLOGÍA DE DIABETES MELLITUSTIPO 1
- La diabetes mellitus tipo 1 (T1D) es un desorden heterogéneo con etiología multifactorial.
- Para su susceptibilidad intervienen tanto factores ambientales como genéticos.
- La participación de los genes se ha documentado ampliamente como poligénica.
- Dentro de estos, la región HLA ha sido descrita como el mayor factor genético de riesgo
BIBLIOGRAFIA:
MICROARRAY
GENE EXPRESSION ANALYSIS FOR TYPE-2 DIABETES
MELLITUS – A STUDY ON DIABETES WITH AND
WITHOUT PARENTAL HISTORY.
Microarray Technology
The scientific principle underlying microarray technology is complementary hybridization between nucleic acids [3,4]. All gene expression DNA microarrays can be understood as highthroughput 'dot-blot' systems, where pieces of known DNA are anchored to a solid support, while targets are fluorescently labeled, free-floating 44 amplified RNA or complementary DNA (cDNA) species originating from the samples [5]. When the labeled sample is hybridized to the DNA microarray, each probe binds its complementary target. Analyzing the microarray with highresolution fluorescent scanners allows assessment of the fluorescent signal strength that originates from the probe-bound target. This signal is presumed to be directly proportional to the abundance of the RNA species present in the investigated samples. BIBLIOGRAFÍA:
http://www.jatit.org/volumes/research-papers/Vol27No1/6Vol27No1.pdfhttp://www.mun.ca/biology/scarr/cDNA_microarray_Principle.jpg
